Strain bj3505
Webacidocalcisome phosphate homeostasis Introduction Phosphate is a limiting factor for the growth of living organisms. It is mainly taken up by the cells as P i and incorporated in biological molecules, including ATP, nucleic acids, and phospholipids. Strain BJ3505 transformed with YEp MEL1 His was grown for 96 h in 1 L YPHSM before being centrifuged (7000 rpm for 10 min, 4 °C), and the supernatant was filtered through 0.45 µm nitrocellulose membrane filters (Millipore) before being dialysed and concentrated by tangential filtration (TFF, Millipore) … See more To select the best secretion and purification system for the ScAGal protein, different variants of the MEL1 gene were expressed in strain … See more Cell-free BJ3505/YEpMEL1His culture medium was concentrated and partially purified by ultrafiltration. The extracellular protein was then … See more ScAGal not only has strong resistance to treatment with all the proteases tested, but also a higher enzymatic activity in their presence (Fig. 3a). … See more In order to assess the best method of conserving the enzyme, a stability study was carried out in cold and environmental conditions (Additional … See more
Strain bj3505
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WebDownload scientific diagram Extra- and intracellular β -galactosidase production by S.cerevisiae strain BJ3505 transformed with plasmid YEpFLAG1-LAC4 ( A) or YEpFLAG1-BC ( B) growing in YPHSM ... Web1 Dec 2003 · Batch fermentations were carried out to examine and compare the patterns of cell growth and sugar metabolism for the two recombinant S. cerevisiae BJ3505 strains, δXR and δXR/STH. As shown in Fig. 1, the expression of the STH gene did not make significant changes in glucose utilization, cell growth, ethanol accumulation, and xylitol …
Web1 Sep 1999 · One strain (BJ3505) has a deletion in the gene encoding the major vacuolar proteases and therefore accumulates catalytically inactive pro-alkaline phosphatase. The … Web10 Oct 2024 · S. cerevisiae strain BJ3505. Different constructions were designed to obtain the degr ee of purification necessary for enzymatic characterization and to improve the …
WebThe protein Ixr1p fused to the FLAG ™ peptide at the carboxyl-terminal end was expressed in the yeast strain BJ3505 . Cells were grown at 30 °C beyond 48 h in YPHSM medium (8% Bacto peptone, 1% yeast extract, 3% glycerol and 1% dextrose) until OD 600 nm 6.0, coinciding with the maximum expression. Cells were lysed in TBS buffer (50 mM Tris ... Web1 Dec 2012 · BJ3505 CBP (calmodulin-binding peptide)–Vam3 nyv1Δ was used for trans-SNARE complex isolation (Table 1). YBT1 was deleted from BJ3505, DKY6281 and …
Web15 Mar 2003 · The plasmid was transformed into yeast strains BJ3505 and DKY6281 using the URA3 marker. For the construction of Vtc3-GFP, GFP was chromosomally integrated …
Web27 Jul 2015 · In brief, strain BJ3505 was grown to mid-log phase in 4 liters of YPD medium. Cells were harvested, washed once with PS buffer containing 150 mM KOAc and 5 mM MgOAc, resuspended in the same buffer containing 1 mM PMSF and 10 µM leupeptin, and quick-frozen by drop-wise addition to liquid nitrogen. The frozen cells were mixed with … flights from lax to bentonville arWebExtra- and intracellular β -galactosidase production by S.cerevisiae strain BJ3505 transformed with plasmid YEpFLAG1-LAC4 ( A) or YEpFLAG1-BC ( B) growing in YPHSM … flights from lax to bhamWeb21 Aug 2007 · Standard fusion and lysis reactions (×16 scale) bore vacuoles from BJ3505 GFP and DKY6281 or 4SNARE-overproducing strains BJ3505 4SNARE ++ GFP, DKY6281 … chernal