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Slowfade gold® antifade reagent invitrogen

Webb14 feb. 2024 · Tissues were washed three more times and stained with 1X DAPI (Cat# 62248; Thermo Fisher) in TBS for 10 min and mounted on microscope slides with cover slips and SlowFade TM Gold Antifade Mountant media (Cat# S36937; Invitrogen). WebbIt contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal.

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Webb20 mars 2008 · The Invitrogen Corporation markets the SlowFade Gold® Antifade Reagent which is an improved version of their original SlowFade® Reagent. SlowFade Gold® was designed to increase the photostability of many different fluorophores used when … WebbProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, … ion chamber commissioning https://brain4more.com

SlowFade™ Glass Soft-set Antifade Mountant - Thermo …

WebbThermo Fisher offers several fluorescent plus biotinylated phalloidin and phallacidin derivatives to labeling F-actin. WebbAMPERE Short Site of the NIH; Biologics Order and Research: The People also Work of Buildings 29 & 29A; Canyon Creek Schoolhouse Labs 100 th Anniversary WebbFixed cells were washed 3 times in blocking solution, 10% bovine serum albumin in PBS, incubated overnight with UCP1 antibody, washed, and incubated with secondary antibody conjugated with FITC. Cells were washed 3 times in PBS and mounted in SlowFade Gold Antifade Reagent with DAPI for nuclear staining (Invitrogen). ontario hockey league dukes of hamilton

ProLong™ Gold Antifade Mountant - Thermo Fisher Scientific

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Slowfade gold® antifade reagent invitrogen

ProLong™ Gold Antifade Mountant - Thermo Fisher Scientific

WebbSlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence … For superior antifade protection of fluorescent proteins and fluorescent … For superior antifade protection of fluorescent proteins and fluorescent … 301 Moved Permanently. nginx/1.20.1 QuantiGene Plex Data Analysis provides differential gene expression analysis on … SlowFade™ Gold Antifade Mountant with DAPI Invitrogen™ SlowFade Gold … Careers at Thermo Fisher Scientific Thermo Fisher Scientific jobs ... home Some apps may appear in the current design, but you'll receive updates as they … Support How can we help? Choose an option. Contact Us WebbThe final wash was applied, and sections were mounted with the addition of an antifade reagent (SlowFade Gold Antifade Reagent, Invitrogen, Carlsbad, CA, USA), and stored until observation in a laser-scanning confocal microscope (Nikon NIS-Elements Eclipse TE2000E, Nikon Corp, Tokyo, Japan).

Slowfade gold® antifade reagent invitrogen

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WebbAbstract. Diabetic cardiac fibrosis increases ventricular stiffness and promotes diastolic dysfunction. The transient receptor potential ankyrin 1 (TRPA1) has been reported to be associated with the occurrence and development of various cardiovascular diseases. WebbInvitrogen™ SlowFade™ Gold Antifade Mountant with DAPI. Protects fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. Manufacturer: Invitrogen™ S36938. View more versions of this product. Catalog No. S36938.

WebbSlowFade® Gold and SlowFade® Diamond Antifade Mountants are intended for short-term use (3–4 weeks); fluorescence signal from samples mounted using this reagent may degrade over time. Technical specifications for SlowFade® Gold and SlowFade® Diamond Antifade Mountants • pH 7.4 at 20°C • Refractive index is 1.42 at 20°C Experimental ... WebbProLong Gold and SlowFade Gold Antifade Reagents - Invitrogen. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian česk ...

WebbAntifade Reagent ‡ SlowFade® Gold antifade reagent (Component G) 2 mL NA • ≤–20˚C • protect from light Reagents for Purifying Labeled Nucleic Acid Binding buff er (Component B) 6.0 mL NA • ≤25˚C Wash buff er (Component C) 3.2 mL NA Elution buff er (Component D) 6.0 mL NA Spin columns and collection tubes (Component E) 20 ... Webb化工仪器网为您提供invitrogen 价格表【第15001条-第16000 ... s36937 slowfade gold antifade reagent 5 x 2 ml 1339.8. s36938 slowfade gold antifade reagent 10 ml 1293.6. s36939 slowfade gold antifade reagent 5 x 2 ml 1456. s37200 sulfate latex, 8% w/v 0.02 um 15 ml 1753.5.

WebbOptional: antifade reagent (ex. ProLong Gold or SlowFade Gold reagent) Preparing the DAPI stock solution To make a 5 mg/mL DAPI stock solution (14.3 mM for the dihydrochloride or 10.9 mM for the dilactate), dissolve the contents of one vial (10 mg) in …

WebbSlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence … ontario hockey league hamilton red wingsWebb7 dec. 2006 · Reagent Setup 0.3% (vol/vol) PBT Add 1.5 ml Triton-X 100 to 498.5 ml PB. Store this nonhazardous buffer at room temperature (20–25 °C). Different staining procedures or antibodies may require... ion chamber designWebb9 dec. 2024 · Also, anti-V5 (R96125), Mito Tracker (M7512), goat serum (50062Z), ProLong Gold antifade reagent with DAPI (P36935) and ViraPower Lentiviral packing mix (K4975-00) from Invitrogen. Anti-ß-actin (A1978 ... and then mounted with Slowfade Light Antifade Kit (Invitrogen). All samples were measured by immunofluorescent microscopy in the ... ion chalk bag small phenomWebb31 dec. 2024 · Cells were then washed twice with HBSS and fixed using 2% paraformaldehyde-HBSS for 15 min at 37 °C. Fixed cells were washed three times with HBSS, and coverslips were mounted onto glass slides using ProLong Gold Antifade reagent (Invitrogen, Australia). Coverslips were examined using a Leica confocal … ion chalk bagWebbThe cochleae were immediately immersed in ice cold 500 μL Trizol Reagent (Invitrogen/Ambion) ... tissue underwent final trimming to ensure the specimens were flat and then coverslipped with SlowFade Gold with DAPI (Invitrogen, ... Wholemount cochleae were mounted on microscope slides with antifade FluoroGold with DAPI (Pierce/Fisher, … ion chalk bag largeWebbInvitrogen™ ProLong™ Gold Antifade Mountant with DNA Stain DAPI Catalog number: P36931 Related applications: Cellular Imaging Technical Support Customer Service ProLong Gold Antifade Mountant with DNA … ion channel activationWebb23 feb. 2024 · The slides were washed three times in PBTr, incubated with FITC-, TRITC-coupled secondary antibodies, or with Alexa-fluor 488/562 (donkey anti-rabbit), diluted in PBTr for 60–75 min at RT, washed three times with PBTr, then two times with PBS followed by mounting with SlowFade Gold antifade reagent with DAPI (Invitrogen, USA), sealed … ontario hockey league brantford alexanders