Hoechst 33342 solution

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August undefined, 2024

NettetAdd a sufficient volume of Hoechst 33342 Working Solution to completely cover the sample. Place aluminum foil over the sample to protect it from light and incubate at … NettetHoechst 33342 solution, 1 mg/ml in water - CAS-Number 23491-52-3 - Order from Chemodex Inquiry Hoechst 33342 solution, 1 mg/ml in water SKU B0447 Category: Fluorescent Detection Synonyms 2'- (4-Ethoxyphenyl)-5- (4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride , BisBenzimide H 33342 , HOE 33342 23491-52-3 …

Hoechst 33342 - an overview ScienceDirect Topics

NettetbisBenzimide H 33342 trihydrochloride. Synonym (s): 2′- (4-Ethoxyphenyl)-5- (4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, … NettetIncubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. 4. Aspirate the labeling solution. Rinse the cells three times in PBS +. 5. Mount the coverslips as described in Mounting Live Cells onto Microscope Slides (Chazotte 2011b). 6. Image the cells (λ ex ~353 nm, λ em ~483 nm for Hoechst 33342). the shine shop gp

Hoechst 33342 Sigma-Aldrich

NettetBlue = Hoechst 33342 (fluorescent DNA dye). Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP ® Rabbit mAb #53348 and Hoechst 33342 (DNA content). Anti-rabbit IgG (H+L), F … NettetHoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced Salt Solution, Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).DMEM+ (Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).. Propidium Iodide … NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used … my singing monsters composer theme songs

Glucose deprivation promotes apoptotic response to S1 by …

Thermo Scientific™ Hoechst 33342 Solution (20 mM) - Fisher Sci

NettetDescription: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, … Nettet26. feb. 2024 · Hoechst 33342, 20 mM solution in water - 5 ml Labeling-detection Hoechst 33342, 20 mM solution in water - 5 ml €97.00 Check your price Cat.Number : AS-83218 Availability : In production Shipping conditions : Ice delivery fees must be applied Select specification Quantity Add to Cart Add to wishlist In Stock Reminder … the shine shop automotive greensboro ncNettetMaterials and methods: Earle’s balanced salt solution (EBSS) was used to simulate glucose deprivation. MTT assay was used to analyze the cell survival rate, and Hoechst 33342 dye was used to detect the apoptosis in HeLa cells. Western blotting was used to detect the expression of ER stress and autophagy relative proteins. the shine shop car wash waukee

"NettetThe Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the … " - Hoechst 33342 solution

Hoechst 33342 solution

NettetBisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. [ 1] This … NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … Features of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent st… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes These dyes can also stain Gram-positive and Gram-negative bacteria. There are … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit contai… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes

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NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … NettetH342 -Cellstain- Hoechst 33342 solution. CAS No. 23491-52-3(Hoechst 33342:free base) Chemical name. ... Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively. Chemical Structure.

NettetHoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP. Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow … NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish …

NettetBio-Rad's PureBlu™ Hoechst 33342 Dye is ideal for routine nuclear staining in fluorescence microscopy, cell imaging applications and cell cycle analysis in flow cytometry. It is available as a ready-to-reconstitute, highly pure powder form, with only one dilution step required to obtain a ready-to-use solution. Hoechst 33342 Binding NettetThe BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA and nuclei in live or fixed cells. Hoechst 33342 is a bisbenzimidazole dye with …

NettetProduct number: 910-3015 Size: 1 ml Storage: 2 – 7°C Solution 15 – Hoechst 33342 is a staining reagent containing Hoechst 33342, which stains the nuclei of living, fixed and permeabilized cells. Solution 15 – Hoechst 33342 is a cell permeable dye that preferably stains doublestranded DNA.

NettetPureBlu Hoechst 33342 Nuclear Staining Dye is provided in an easy-to-reconstitute format. Each vial contains 56 μg of PureBlu Hoechst 33342 powder to generate 50 ml of 1.1 μg/ml (2 μM) working solution. Property Description Formula C 27 H 28 N 6 O • 3HCl Molecular weight 561.93 Maximum excitation/emission 350 nm/461 nm CAS 23491-52-3 the shine shop ankeny iowaNettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are maximally excited around 355 nm and emit around 460 nm. A UV light source is required, which may harm the cell. my singing monsters composer sheets the shine restaurant conway scNettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … my singing monsters congleNettetNote: Both BD Pharmingen™ Hoechst 34580 and BD Pharmingen™ Hoechst 33342 Solution (Cat. No. 561908) may be used interchangeably for most applications according to the Recommended Assay Procedure for each dye. the shine shop elk groveNettet15. sep. 2024 · Hoechst 33342 Staining Dye Solution 是一种用于在荧光显微镜下标记 DNA 的荧光染料。该产品可用于荧光显微镜、微孔板、比色皿和流式细胞仪应用。它还可用于通过绘制标准排放-含量曲线来检测样本 DNA 的含量。 hoechst 33342染色原理 Hoechst 33342 渗透细胞膜并与富含 A/T 的 dsDNA 序列的小沟结合，从而优先染色细 … my singing monsters composer tutorialsNettetThermo Scientific™ Hoechst 33342 Solution (20 mM) High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques. … my singing monsters connection issues