Hcs lipidtox
WebHCS LipidTOX Red solution from the manufacturer µl/10 ml PBS). Cells in MatTek dishes were stained after fixation by addition of 2 ml solution of dye in PBS. To reduce the effects of temperature variation on stain-ing efficiency, we incubated the fixed cells in staining solution for 30 min at 37C. They were then immedi- WebApr 28, 2016 · 2μL ethidium homodimer (LIVE/DEAD Viability/Cytotoxicity Kit; Molecular Probes) and 1μL of HCS LipidTOX Deep Red (Molecular Probes) was mixed into each well containing placental explants. After 30mins, 100μL of 10μM BODIPY-C 12 solution was added to each well and mixed by trituration (total well volume 500μL, final concentration …
Hcs lipidtox
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WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. Description; Category: Stain; MW: N/A. Stock Concentration: 1000 X. Preparation; Solvent for Stock Solution: DMSO. Storage; Temperature-20°C; Precaution(s) Protected from light; WebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … WebInvitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging. High affinity for neutral lipid droplets. Brand: Invitrogen™ H34475. Code : 50. Additional Details : …
Webthe Invitrogen ™ HCS LipidTOX neutral lipid stains were developed to detect intracellular lipid droplets or globules and characterize the effects of drugs and other compounds on lipid metabolism in mammalian cell lines. These reagents are added after cell fixation (Figure 5), Figure 2. Real-time monitoring of the formation of OxLDL. WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. …
WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically to amplify lipid accumulation in the observed puncta. We note that the most significant increases in DUNN index were …
images of marilyn manson without makeupWebDec 7, 2024 · Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of … images of marilee fiebigWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … images of marina ca