WebDesigned for use in immunofluorescent staining protocols of cells in suspension. A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a … WebStop cell lysis by adding 10ml Cell Staining Buffer to the tube. Centrifuge for 5 minutes at 350xg and discard supernatant. Repeat wash as in step 2. Count viable cells and resuspend in Cell Staining Buffer at 5-10 x 10 6 cells/ml and distribute 100µl/tube of cell suspension (5-10 x 10 5 cells/tube) into 12 x 75mm plastic tubes.
R718 Mouse Anti-Human Myeloperoxidase
WebUse standard staining buffer with UltraComp beads when staining single-color compensation tubes with Super Bright-conjugated antibody. Super Bright Complete Staining Buffer is compatible with Super Bright and Brilliant Violet fluorochromes when both are used for staining in the same tube of cells.) ... Flow Cytometry Facility 431 Newton … WebDec 18, 2024 · Centrifuge at 400 × g for 5 min at 4°C, discard the supernatant, and resuspend the pellet with 2 mL cold Staining Buffer. 26. Centrifuge at 400 × g for 5 min at 4°C, discard the supernatant, and resuspend the pellet with 90 μL cold Staining Buffer. 27. Transfer the sample to a 5-mL round-bottom flow test tube. Keep on ice until staining. how to draw a cupped hand
Staining Protocols Flow Cytometry - University of Iowa
WebInvitrogen™ Accutase™ Enzyme Cellular Detachment Medium (cat. no. 00-4555) instead trypsin other ethylenediaminetetraacetic acid (EDTA) ... Centrifuge cells how in Step 4 … WebSample preparation reagents for flow cytometry include cell surface staining, intracellular and transcription factor staining buffer sets, cell lysis assays, blocking reagents, and … WebAdd 2 mL of Flow Cytometry Staining Buffer to each tube and centrifuge at 400-600 x. g. for 3-5 minutes. 8. Decant supernatant and add 0.2-0.4 mL of Flow Cytometry Staining Buffer to each tube. ... Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of ... how to draw a curled up fox