WebCrude Protein Determination in Feed and Forages Macro-Kjeldahl Method 1. Application This procedure is applicable for the determination of nitrogen (N) in forage. Crude protein is derived through a calculation using this nitrogen value. 2. Summary of Methods The Kjeldahl method is the standard method of nitrogen determination. ... WebApr 14, 2024 · Crude protein was determined by the macro-Kjeldahl method using Kjeldahl Apparatus (BUCHI, USA). Ash content was measured by weighing the sample after it was subjected to heat treatment at a temperature of 500 °C in a muffle furnace. The determination of ether extract or crude fat was done by the ether extraction method.
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WebSep 23, 2024 · This editorial provides an overview of different protein determination methods and describes their advantages and disadvantages. ... Crude protein analysis … WebDec 31, 2024 · There was an increase in crude fibre for infested Clariidae and Characidae while uninfested and infested had Protopteridae and Mormyridae had no crude fibre. There were no significant difference, (P 0.05) in the moisture and protein. The selected fish samples had weight loss and nutritional loss in all the agricultural zones of Benue state. temperature for black tea
PROTEIN DETERMINATION BY KJELDAHL METHOD
WebCrude protein (%) = nitrogen in sample × 6.25. Where: A = chlorhydric acid used in titration (ml) B = normality of standard acid C = weight of sample (g) Figure 2. Determination of crude protein by Kjeldahl's method. 3.2.2 Standard MAFF (1982) method for determining proteins in feedstuffs and feed ingredients. Reagents. Mercuric oxide. WebCrude protein is a term used to describe the total protein content of a grain, a grain product or a food when the protein measurement is done by determining it nitrogen content. ... Combustion method for determination of crude protein. Results are reported as a percentage, N x 6.25, calculated to specified moisture basis. Canola is calculated ... WebFeb 28, 2024 · The Kjeldahl method is an AOAC official method for crude protein content and has been the basis for evaluation of many other protein methods. The Kjeldahl method is still used for some applications, but nowadays its use is limited in many countries due to the availability and advantages of automated nitrogen combustion (Dumas) systems. temperature for black powder to explode