Web-DCPIP is reduced - it accepts electrons from chlorophyll in LD reaction -instead of NADP accepting the electrons, meaning NADP is not reduced so less is produced & less ATP as LI & LD is happening less than usual. RP 8 - Method? (5) 1. Put isolation medium into a beaker (which has the same water potential as chloroplasts). 2. WebPut 5 cm3 DCPIP solution + 1 cm3 water + 1 cm3 isolation medium in the tube. Tubes A and B are control experiments. Leave both tubes until the end of your investigation. 9. Set up tube C as follows: Tube C Put 6 cm3 water + 1 cm3 chloroplast suspension in the tube.
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WebThe use of DCPIP and other electronacceptors in redox reactions can be discussed. Redox reactions are reactions where red uction and ox idation take place. This will allow … WebPART 1: Isolation of Chloroplasts (cont) 5. Remove the supernatant and centrifuge it at 100 xg for 10 minutes. 6. Collect the precipitate and suspend it in 10 ml of cold reaction medium (0.01M KCl in 0.03M phosphate buffer, pH 6.5). 7. Stir and store in an ice bath until needed. This is the chloroplast suspension. nyu wagner admissions
RP8: Investigation into the effect of a named factor on the rate of ...
WebThe reagents and medium were sterilized; 750 µL of BHM, 50 µL of FeCl 3 ⋅6H 2 O solution (150 µg/mL) and 50 µL of 2,6-DCPIP solution (37.5 µg/mL) were taken in a 1.5 mL tube. About 80 µL of cell suspension and 5 µL of crude oil were added to this tube and incubated at 30 °C and 100 r/min for 48 h. WebEl presente trabajo tuvo como objetivo determinar la degradación del aceite lubricante por Pseudomonas aeruginosa. En primer lugar, elcultivo fue proporcionado por el Laboratorio de Biotecnología e Ingeniería Genética de la Universidad Nacional de Trujillo, se realizó lareactivación y verificación de la pureza del cultivo, luego se procedió a la evaluación … WebAn isolation medium (usually made up of a phosphate buffer solution, sucrose, and potassium chloride). Make sure this is ice cold so that the chloroplasts are not damaged. … nyu waive insurance