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Cisplatin ic50 a549

WebApr 11, 2024 · A549 and H358 cells were seeded in 6-well plates for 24 h prior to transfection with siRNA targeting BATF3, IRF5, ZBTB38, and corresponding non-targeting controls. A total of 150 nM of siRNA was added to each experiment made up of the target siRNA and topped up with the appropriate concentration of non-targeted controls where … WebThe IC50 value of cisplatin was also assessed with CCK-8 assays. The western blotting for AKT, p-AKT, S6K and p-S6K were performed in cells with TAZ knockdown or overexpression. When the AKT/mTOR pathway was blocked in A549 and H460 with TAZ overexpression, cisplatin sensitivity was assessed by IC50 value.

Circular RNA hsa_circ_0096157 contributes to cisplatin resistance …

WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … WebAug 6, 2024 · The half-maximal inhibitory concentration (IC50) of A549 and A549/DDP cells was determined using CCK-8 assay. Further, circRNA profiles and differentially expressed genes in A549 and A549/DDP cells were characterized by deep sequencing and cell proliferation was measured using MTS assay. Cell cycle progression was analyzed using … tack door latch https://brain4more.com

Cisplatin Induces a Mitochondrial-ROS Response That Contributes …

WebMar 22, 2024 · The IC50 (drug concentration producing 50% growth inhibition) was calculated with GraphPad Prism Version 5.0 Software. Cell proliferation assay Cell … WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for … WebDec 29, 2009 · Fig. 3B confirms that, unlike cisplatin, a conventional chemotherapeutic agent, mitaplatin selectively induced cell death in human cancer A549 cells, but not in normal MRC-5 cells under the similar treatment conditions. To address whether the selective killing of cancer cells by mitaplatin might be a consequence of its selective uptake, we ... tack donations

Cisplatin-resistant A549 non-small cell lung cancer cells …

Category:TAZ inhibition restores sensitivity of cisplatin via AKT/mTOR

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Cisplatin ic50 a549

Through oxaliplatin resistance induction in colorectal cancer …

WebMar 17, 2024 · To establish the cisplatin-resistant cell line, A549-R, A549 cells were treated with a stepwise increasing concentration of cisplatin (Sigma-Aldrich, USA) as previously described. 29, 30 The CAFs and normal fibroblasts (NFs) were isolated from NSCLC tissues and adjacent normal tissues by primary culture, as previously described, respectively. … WebWith the treatment prolongation of 4 μmol/L Cisplatin in A549 cells, Bcl-2 and caspase-3 were downregulated, while BAD upregulated. Conclusions: Cisplatin treatment induces …

Cisplatin ic50 a549

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WebFeb 3, 2024 · A549 and A549/CDDP cells were treated with 1/2 IC50 of cisplatin and/or PD‐0332991 for 24 h. Then cells were homogenized in RIPA buffer (50 mM Tris–HCl, pH … WebJan 3, 2003 · We used the IC50 concentrations for both Gleevec and cisplatin to treat A549 cells in culture, and assessed the viability of the cells by MTT assays at the different time points (Figure 4 ). Gleevec (2 μM) and cisplatin (64 μM) both inhibited the growth of the tumor cells individually after treatment of the cells for 48 hour.

WebJan 3, 2003 · The concentration of Gleevec to inhibit 50% cell growth (IC50) was estimated to be around 2–3 μM (Figure 1), whereas the IC50 of cisplatin on A549 cells was … WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were performed to reveal the relative mobility. The line graphs show the mean ± SD of three scratches from one representative experiment.

WebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... WebNext, the highest cisplatin IC50 was found in CAFs, and A549 cells had the lowest IC50 value . The ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ...

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WebJan 3, 2003 · We have also shown that A549 cells are resistant to cisplatin treatment (IC50 64 microM). Addition of Gleevec to the A549 cells treated with cisplatin resulted in a synergistic cell killing effect, suggesting that Gleevec can potentiate the … tack down stitchWebMar 22, 2024 · The drug’s IC 50 values indicated that the cells had acquired a stable CDDP-resistance phenotype, with the IC 50 values of the A549/DDP cells remaining … tack e tmi italy s.r.lWebMay 11, 2016 · A549 cells. After 156 hrs of monitoring, the diameter of A549 spheroid controls decreased by a total of 141 μM. During that time, spheroids treated with 20 and 40 μM of DXR also decreased, shrinking by −206 μM and −178 μM, respectively, the rate of shrinkage was faster in the treated spheroids compared with the controls. tack etf fairleadWebThe IC50 values are slightly higher than those previously reported in the literature for cisplatin, 5.7 ± 0.1 (incubation for 24 h) and 14.4 ± 1.1 (incubation for 48 h) for MCF-7 [64] and PANC-1 ... tack down stitch on brother sewing machineWebApr 8, 2024 · Lung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated the role of mTOR signaling and other signaling pathways after metformin (MET) treatment in control and cisplatin‑resistant A549 cells, mapping pathways and possible … tack edmontonWebIn A549 cells, the IC50 concentration of cisplatin resistant cells was determined as 23.60 µM compared to 1.58 µM in the original parent cell line, a 15-fold increase in the … tack down embroidery designWebIC 50 values for cisplatin, curcuminoid, and combination treatments in A549 cell culture. Standard deviation values are provided after the ± symbol for each inhibitory concentration value. Cell... tack electronics indeed